Your collection file is too large to send to AnkiWeb. You can reduce its size by removing any unwanted decks (optionally exporting them first), and then using Check Database to shrink the file size down. (⁨485MB > 250MB⁩)

Here’s a list of things to look at to get your collection size down under the limits – Constant Errors Uploading / Sync - #4 by Danika_Dakika .