The app is signed and notarized, so changing the security settings from the defaults should not be necessary. I’d recommend against selecting ‘anywhere’ unless you’re planning to change it back immediately after testing. @Nickie, are you using one of the new Apple Silicon Macs? Is your macOS version fully up to date, or are there updates pending that you haven’t installed yet? This issue may be related to Anki App will not open - #6 by Alison